No wash, just go: Classical antibody-detection methods rely on heterogeneous detection schemes that involve multiple, time-consuming binding and washing steps. Here we present a new concept to translate the antigen–antibody interaction directly into a readily detectable fluorescent signal by using a single-chain sensor protein and taking advantage of the unique Y-shaped structure common to all antibodies.
No wash, just go: Classical antibody-detection methods rely on heterogeneous detection schemes that involve multiple, time-consuming binding and washing steps. Here we present a new concept to translate the antigen–antibody interaction directly into a readily detectable fluorescent signal by using a single-chain sensor protein and taking advantage of the unique Y-shaped structure common to all antibodies.
