澳门太阳游戏城app官网入口

至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Extracellular and macropinocytosis internalized ATP work together to induce epithelial-mesenchymal transition and other early metastatic activities in lung cancer

Cancer Cell Int. 2019; 
Cao Y, , Wang X, , Li Y, Evers M, Zhang H, , Chen X, , .
Products/Services Used Details Operation
Gene Synthesis … The fluorescence images were taken using a fluorescence microscope (ECLIPSE E600, Nikon). CRISPR–Cas9 mediated gene knockout. CRISPR guide RNA (gRNA) sequence design and plasmid preparation were provided by GenScript (Piscataway, NJ) … Get A Quote

摘要

Extracellular ATP (eATP) was shown to induce epithelial-mesenchymal transition (EMT), a very important early process in metastasis, in cancer cells via purinergic receptor signaling. However, the exact induction mechanisms are far from fully known. We previously described that eATP is internalized by cancer cells in vitro and in vivo by macropinocytosis in human non-small cell lung cancer A549 and other cancer cells, drastically elevates intracellular ATP levels, enhances cell proliferation and resistance to anticancer drugs. In this study, we tested the hypothesis that eATP and macropinocytosis-internalized eATP also induces EMT and other early steps of metastasis.,Floating cells, fencing, and transwell assays... More

关键词