Impact of novel Caveolin-1 frameshift mutants on caveolae assembly and function
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| Gene Synthesis |
The protein sequence of P. fucata Pif80 was obtained from the work of Suzuki et al. (15). The gene encoding Pif80 was optimized for protein expression by adjusting the codon usage and frequency to match the preferences of E. coli. The target gene was constructed with the addition of N-terminal Nde I and C-terminal Xho I restriction sites (GenScript USA Inc.) and was inserted into a pET-22b(+) vector (Novagen), resulting in pET22-rPif80. T |
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