is an important medicinal plant widely used in traditional Chinese medicine. Luteoloside is a critical bioactive compound in . To date， the molecular mechanisms underlying luteoloside biosynthesis are still largely unknown. In this work， high performance liquid chromatography (HPLC) was employed to determine the luteoloside contents in leaves， stems， and flowers at different developmental stages. Results showed that senescing leaves can accumulate large amounts of luteoloside， extremely higher than that in young and semi-lignified leaves and other tissues. RNA-Seq analysis identified that twenty-four differentially expressed unigenes (DEGs) associated with luteoloside biosynthesis were significantly up-regulated in senescing leaves， which are positively correlated with luteoloside accumulation. These DEGs include ， ， thirteen ， ， six () and two () genes. Further analysis demonstrated that two ( and ) and two ( and ) might play vital roles in luteoloside generation. Furthermore， several transcription factors (TFs) related to flavonoid biosynthesis including MYB， bHLH and WD40， were differentially expressed during leaf senescence. Among these TFs， ， ， and were considered to be key factors involved in the regulation of luteoloside biosynthesis. These findings provide insights for elucidating the molecular signatures of luteoloside accumulation in .