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Cloning and Expression of Novel Aminoglycoside Phosphotransferase Genes from Campylobacter and Their Role in the Resistance to Six Aminoglycosides.

Antimicrob. Agents Chemother.. 2018-01; 
ZhaoS，MukherjeeS，LiC，JonesS B，YoungS，McDermot
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Gene Synthesis … were used in this study (Table 1). All isolates were previously sequenced using the Illumina MiSeq (Illumina, San Diego, CA, USA) (1, 4). The coding sequences of nine aph genes were synthesized and cloned in a pBluescript expression vector by GenScript (Piscataway, NJ) … Get A Quote

摘要

Nine genes， including ， ， ， ， ， ， ， -， and -， were previously identified in To measure the contribution of these alleles to aminoglycoside resistance， we cloned nine genes into the pBluescript and expressed them in DH5α. The nine expressed in showed various levels of resistance to gentamicin， kanamycin， and tobramycin. Three genes， -， ， and ， showed increased MICs to amikacin， and five genes were transferrable.

关键词

Campylobacter，NARMS，cloning and expression，gentamicin resist
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