The presence of specific antibodies against human polyomavirus 12， Saint Louis polyomavirus and New Jersey polyomavirus was investigated by using virus-like particle-based ELISAs with serum samples from 706 Italians aged 1- to 100-years-old. The findings indicate that these polyomaviruses circulate widely in humans， with peak seroprevalence， observed at adulthood， of 97.3%， 93.3%， 57.5%， for human polyomavirus 12， Saint Louis polyomavirus and New Jersey polyomavirus， respectively.