MADS-box genes form a large gene family in plants and are involved in multiple biological processes， such as flowering. However， the regulation mechanism of MADS-box genes in flowering remains unresolved， especially under short-term cold conditions. In the present study， we isolated ， a Pak-choi ( ssp. ) ()， as a floral repressor and functionally characterized in and Pak-choi. Subcellular localization and sequence analysis indicated that BcMAF1 was a nuclear protein and contained a conserved MADS-box domain. Expression analysis revealed that had higher expression levels in leaves， stems， and petals， and could be induced by short-term cold conditions in Pak-choi. Overexpressing in showed that had a negative function in regulating flowering， which was further confirmed by silencing endogenous in Pak-choi. In addition， qPCR results showed that expression was reduced and expression was induced in -overexpressing . Meanwhile， transcript was up-regulated and transcript was down-regulated in -silencing Pak-choi. Yeast one-hybrid and dual luciferase transient assays showed that BcMAF1 could bind to the promoters of and . These results indicated that and might be the targets of BcMAF1. Taken together， our results suggested that could negatively regulate flowering by directly activating and repressing .