| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | … The expression levels of each mRNA were calculated using the 2−ΔΔCt algorithm normalizing to GAPHD. All primers were synthesized by GenScript (Nanjing, China). The sequences of primers were as follows: Probe name, Sequence 5′-3 … | Get A Quote |
Zika virus (ZIKV) can lead to severe birth defects especially microcephaly in newborns by infecting human neural progenitors and impairing brain development. However， as the resident immune cells in the brain， the role of microglia in the ZIKV pathology is not clearly defined. To understand the interplay between immune response and neural cells， we investigate the interaction between microglia and NPCs during ZIKV infection. Our results demonstrate that primary microglia infected with ZIKV induces an inflammatory response similar to that in human， producing high level of tumor necrosis factor alpha (TNF-α)， interleukin 6 (IL-6)， interleukin 1β (IL-1β) and inducible nitric oxide synthase (iNOS). F... More
