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Purification of therapeutic proteins mediated by in vivo polyester immobilized sortase.

Biotechnol. Lett.. 2018-02; 
DuJinping，RehmBernd
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Gene Synthesis … Plasmid construction. Plamids pET14b-PhaC-SrtA-TNFα and pET14b-PhaC-SrtA-IFNα2b were prepared as previously described with tnfα and ifnα2b gene being ordered from Genscript (Hay et al. 2015b). PHA bead isolation via microfluidizer … Get A Quote

摘要

To overcome laborious and costly procedures often associated with therapeutic protein production and purification， in vivo polyester immobilized sortase is explored for the production of human tumor necrosis factor alpha (TNFα) and human interferon alpha 2b (IFNα2b) by Escherichia coli.

关键词

Polyhydroxyalkanoate，Polyhydroxyalkanoate synthase，Protein purification，Self-cleavage，Sortase，Therapeutic prot
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