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Episomal HBV persistence within transcribed host nuclear chromatin compartments involves HBx.

Epigenetics Chromatin. 2018-01; 
HenselKai O，CantnerFranziska，BangertFelix，WirthStefan，Postber
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Gene Synthesis … To study the nuclear localization and HBV episome-binding properties of nuclear HBx, constructs with an N-terminal nuclear localization signal (NLS) were synthesized (Genscript, Piscataway, NJ, USA) and subsequently cloned into the pTagRFP-N vector (Evrogen, Moscow … Get A Quote

摘要

In hepatocyte nuclei， hepatitis B virus (HBV) genomes occur episomally as covalently closed circular DNA (cccDNA). The HBV X protein (HBx) is required to initiate and maintain HBV replication. The functional nuclear localization of cccDNA and HBx remains unexplored.

关键词

Chromatin fiber loops，Epigenome，Episome，HBxAg，Host–pathogen interaction，Oncogene，Supranucleosomal structure，TADs，Transcription factories，X-pro
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