| Products/Services Used | Details | Operation |
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| Gene Synthesis | … Control (mock transfected) cells were exposed to the transfection mixture with no plasmid DNA. The plasmid vector pcDNA3.1+P2A-eGFP coding for Kv 3.1 (KCNC1, NCBI Reference Sequence: NM_004976.4) was purchased from GenScript (USA) … | Get A Quote |
In the human genome， more than 400 genes encode ion channels， which are ubiquitously expressed and often coexist and participate in almost all physiological processes. Therefore， ion channel blockers represent fundamental tools in discriminating the contribution of individual channel types to a physiological phenomenon. However， unspecific effects of these compounds may represent a confounding factor. Three commonly used chloride channel inhibitors， i.e. 4，4'-diisothiocyano-2，2'-stilbene-disulfonic acid (DIDS)， 5-nitro-2-[(3-phenylpropyl) amino]benzoic acid (NPPB) and the anti-inflammatory drug niflumic acid were tested to identify the lowest concentration effective on Cl- channels and ineffecti... More
