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MNT Optimization for Intracellular Delivery of Antibody Fragments.

Dokl. Biochem. Biophys.. 2018-03; 
UlasovA V，KhramtsovY V，LupanovaT N，TsvetkovaA D，RosenkranzA A，SlastnikovaT A，GeorgievG P，Sobole
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Gene Synthesis … The cDNA sequence encoding hMyo, which was optimized for the expression in E. coli, was synthesized by Genscript (United States) and cloned into the plasmid [5] encoding 6His–DTox–HMP–NLS–EGF, with the replacement of the HMP module with hMyo … Get A Quote

摘要

We studied the possibility of optimizing modular nanotransporters (MNTs) for the intracellular delivery of antibody fragments into the nuclei of cells of a specified type. Basic MNT with a reduced size retaining the desired functions was obtained， and the principal possibility of obtaining an MNT carrying an antibody fragment by microbiological synthesis was shown.

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