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Effective preparation of a monoclonal antibody against human chromogranin A for immunohistochemical diagnosis.

BMC Biotechnol.. 2018-05; 
ZhangDanping,XieChengjie,WangRongzhi,YangQinghai,ChenHuiling,LingSumei,WangShihua,JiaKu
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Gene Synthesis … The coding part of CgA was selected to optimize codon for expression in E. coli BL21 (DE3) and evaluated by graphical codon usage analyser (http://gcua.schoedl.de) [6]. The optimized DNA of CgA was synthesized by Nanjing Genscript Biotech Co., Ltd. (Nanjing, China) … Get A Quote

摘要

Human chromogranin A (CgA) is a ~ 49 kDa secreted protein mainly from neuroendocrine cells and endocrine cells. The CgA values in the diagnosis of tumor, and in the potential role in prognostic and predictive tumor as a biomarker.

关键词

Chromogranin A,Immunohistochemistry,Monoclonal antibody,Specifility,iE