The aim of this study was to elucidate the hormonal regulation of leptin synthesis by the white adipose tissue (WAT) during the period of fat accumulation in male Scotophilus heathi. An in vivo study showed a significant correlation between the seasonal changes in serum insulin level with the circulating leptin level and with the changes in body fat mass in S. heathi. An in vitro study showed insulin induced a significant increase in expression of leptin protein in WAT. The insulin-stimulated increase in leptin expression was associated with increased uptake of glucose in the WAT. Two glucose transporters (GLUT4 and GLUT8) are utilized for transport of glucose in the WAT during adipogenesis in the bat. The bats showed high insulin and glucose levels, but a reduction in insulin receptor protein during the period of fat deposition, suggesting insulin resistance, which improved in late winter (January) when most of the fat has been utilized as a metabolic fuel. The in vitro study confirmed that insulin enhanced leptin and GLUT4 expression in WAT. The in vitro study further showed that the expression of leptin is directly proportional to the amount of glucose uptake by the WAT. The expression of GLUT4 and GLUT8 were also shown to be differentially regulated by insulin during adipogenesis. The insulin-stimulated increase in leptin synthesis by WAT is mediated through phosphorylation of MAPK in S. heathi. The specific role of GLUT4 and GLUT8 in the regulation of leptin synthesis during adipogenesis needs further investigation.