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MASP2, His, Rat

The dysregulation of complement cascade leads to unsolicited cytokine storm, inflammation, deterioration of alveolar lining cells, culminating in acquired respiratory destructive syndrome (ARDS). Similar pathogenesis is observed with the middle east respiratory syndrome (MERS), severe acquired respiratory syndrome (SARS), and SARS-CoV-2. Activation of the lectin pathway via mannose-binding lectin associated serine protease 2 (MASP2) is witnessed under discrete viral infections including COVID-19.
￥4500
Z06426-100

产品简介
Species Rat
Protein Construction
MASP2 (Thr287-Phe685)_x000D_
Accession # A2VCV7		 His
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System E.coli
Theoretical Molecular Weight 46.7 kDa
Apparent Molecular Weight Due to autocatalytic cleavage, the protein migrates to 15-20 kDa&25-30 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in 50mM Tris, 200mM NaCl (pH 9.0).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
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图例
MASP2, His, Rat

Immobilized MASP2, His, Rat, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA. »

MASP2, His, Rat

The purity of MASP2, His, Rat is greater than 95% as determined by SEC-HPLC. »

MASP2, His, Rat

MASP2, His, Rat on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

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技术背景
Target Background The dysregulation of complement cascade leads to unsolicited cytokine storm, inflammation, deterioration of alveolar lining cells, culminating in acquired respiratory destructive syndrome (ARDS). Similar pathogenesis is observed with the middle east respiratory syndrome (MERS), severe acquired respiratory syndrome (SARS), and SARS-CoV-2. Activation of the lectin pathway via mannose-binding lectin associated serine protease 2 (MASP2) is witnessed under discrete viral infections including COVID-19.
Synonyms MAP19; MASP-2; MASP1P1; sMap
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.