| ￥3300 | |
| Z05905-100 | |
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| Species | Human | ||||||
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | ||||||
| Endotoxin Level | Less than 1 EU per μg by the LAL method. | ||||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 58.2 kDa | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Immobilized TNFSF15 Trimer, His & Flag, Human, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human DR3, hFc Tag with the EC50 of 2.38 μg/ml determined by ELISA. »
Immobilized TNFSF15 Trimer, His & Flag, Human, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. »
Immobilized TNFSF15 Trimer, His & Flag, Human, His Tag at 1 μg/ml(100 μl/well) on the plate. Dose response curve for Human DcR3, hFc Tag with the EC50 of 38.5ng/ml determined by ELISA. »
The purity of TNFSF15 Trimer, His & Flag, Human is greater than 95% as determined by SEC-HPLC. »
TNFSF15 Trimer, His & Flag, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
TNFSF15 Trimer, His & Flag, Human, hFc Tag captured on CM5 Chip via Protein A can bind Human TNFSF15 Trimer, His Tag with an affinity constant of 0.10 nM as determined in SPR assay (Biacore T200). »
TNFSF15 Trimer, His & Flag, Human, His Tag immobilized on CM5 Chip can bind Human TNFSF15 (Trimer) , His Tag with an affinity constant of 3.06 nM as determined in SPR assay (Biacore T200). »
| Target Background | TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2. |
| Synonyms | TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
