| ￥2600 | |
| Z05783-100 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity | > 95% as determined by Bis-Tris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 14.95 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 20-25 kDa and 26-32 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Immobilized R spondin 3/RSPO3, His, Human, His Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA. »
Human R spondin 3/RSPO3, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Human LGR-5, hFc Tag captured on CM5 Chip via Protein A can bind R spondin 3/RSPO3, His, Human, His Tag with an affinity constant of 0.59 nM as determined in SPR assay (Biacore T200) (Routine Test). »
| Target Background | The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein. |
| Synonyms | R-spondin-3; RSPO3; hPWTSR; hRspo3; PWTSR; THSD2 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
