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| Z05728-100 | |
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| Species | Human | ||||||
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | ||||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | OX40/TNFRSF4/CD134, His & Avi, Human captured on CM5 Chip via AntiHis Antibody can bind Human OX40 Ligand, hFc Tag in SPR assay (Biacore T200). Test result was comparable to standard batch. | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 23.2 kDa | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Immobilized OX40/TNFRSF4/CD134, His & Avi, Human, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. »
The purity of OX40/TNFRSF4/CD134, His & Avi, Human is greater than 95% as determined by SEC HPLC. »
OX40/TNFRSF4/CD134, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
OX40/TNFRSF4/CD134, His & Avi, Human, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human OX40 Ligand, hFc Tag with an affinity constant of 4.17 nM as determined in SPR assay (Biacore T200). »
| Target Background | Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation. |
| Synonyms | CD134; OX40; OX40L receptor; TNFRSF4; ACT-135; Ly-70; OX40 homologue; TXGP1L; IMD16 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
