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目录产品 » Notch 1, His & Avi, Human
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Notch 1, His & Avi, Human

Human Notch-1 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes.Notch-1 functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
￥3000
Z05701-100

产品简介
Species Human
Protein Construction
Notch 1 (Ala19-Gln526)_x000D_
Accession # P46531		 His Avi
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE
Endotoxin Level Less than 1EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 56.5 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
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图例
Notch 1, His & Avi, Human

Immobilized Notch 1, His & Avi, Human, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human DLL4, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA. »

Notch 1, His & Avi, Human

The purity of Notch 1, His & Avi, Human is greater than 95% as determined by SEC-HPLC. »

Notch 1, His & Avi, Human

Notch 1, His & Avi, Human on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. »

Notch 1, His & Avi, Human

Human DLL4, hFc Tag captured on CM5 Chip via Protein A can bind Notch 1, His & Avi, Human, His Tag with an affinity constant of 0.48 μM as determined in SPR assay (Biacore T200). »

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技术背景
Target Background Human Notch-1 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes.Notch-1 functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
Synonyms Notch-1; TAN1; NOTCH1; 9930111A19Rik; lin-12; Mis6; N1
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.