| ￥3600 | |
| Z05685-100 | |
|
|
|
|
|
|
|
|
|
| Species | Human | ||||
| Protein Construction |
|
||||
| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 56.9 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 70-90 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Serial dilutions of Anti-NKG2A Antibody were added into Biotinylated Human HLA-E*01:03&B2M&Peptide (VMAPRTLVL) Tetramer, His Tag : NKG2A&CD94 mFc Chimera, Human, mFc Tag binding reactioins. The half maximal inhibitiory concentration(IC50) is 0.15μg/ml. »
Immobilized NKG2A&CD94 mFc Chimera, Human, mFc Tag at 1 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. »
The purity of NKG2A&CD94 mFc Chimera, Human is greater than 95% as determined by SEC-HPLC. »
NKG2A&CD94 mFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
NKG2A&CD94 mFc Chimera, Human, mFc Tag captured on CM5 Chip via Anti-mouse Antibody can bind Human HLA-E Complex Tetramer, His Tag with an affinity constant of 7.90 nM as determined in SPR assay (Biacore T200). »
| Target Background | The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies. |
| Synonyms | CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
