| ￥2600 | |
| Z05673-100 | |
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| Species | Human | ||||||
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | ||||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 38.4 kDa | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 45-52 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Immobilized Nectin-2/CD112, His & Avi, Human, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 0.16μg/ml determined by ELISA. »
The purity of Nectin-2/CD112, His & Avi, Human is greater than 95% as determined by SEC-HPLC. »
Nectin-2/CD112, His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
Human TIGIT, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Nectin-2/CD112, His & Avi, Human, His-Avi Tag with an affinity constant of 1.84 μM as determined in SPR assay (Biacore T200). »
Human PVRIG, mFc Tag captured on CM5 Chip via Protein A can bind Nectin-2/CD112, His & Avi, Human, His Tag with an affinity constant of 87.49 nM as determined in SPR assay (Biacore T200). »
| Target Background | Nectin-2 is an adhesion molecule that has been reported to play a role in tumor growth, metastasis and tumor angiogenesis. Nectin-2 expression in ovarian cancer may support tumor cell adhesion, leading to growth and lymph node metastasis. Effect of VEGF on Nectin-2 expression as well as permeability was investigated in HUVEC. |
| Synonyms | CD112; MPH; Nectin2; PRR2; PVRL2; HVEB; PVRR2 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
