| ￥4000 | |
| Z05142-100 | |
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| Species | Human | ||||
| Protein Construction |
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| Purity | > 95% as determined by BisTris PAGE | ||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||
| Biological Activity | Measured by its binding ability in a functional ELISA. Test result was comparable to standard batch. | ||||
| Expression System | HEK293 | ||||
| Theoretical Molecular Weight | 72.8 kDa | ||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result. | ||||
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). | ||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Loaded Anti-CDCP1 Antibody, hFc-Avi Tag on ProA-Biosensor can bind CDCP1, His, Human, His Tag with an affinity constant of 17.00 nM as determined in BLI assay (Gator® Prime). »
Immobilized CDCP1, His, Human, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. »
The purity of CDCP1, His, Human is greater than 95% as determined by SEC-HPLC. »
CDCP1, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
CDCP1, His, Human, His Tag captured on CM5 Chip via anti-His antibody can bind Anti-CDCP1 Antibody, hFc Tag with an affinity constant of 0.18nM as determined in SPR assay (Biacore T200). »
| Target Background | Tumor metastasis depends on the dynamic regulation of cell adhesion through β1-integrin. The Cub-Domain Containing Protein-1, CDCP1, is a transmembrane glycoprotein which regulates cell adhesion. Overexpression and loss of CDCP1 have been observed in the same cancer types to promote metastatic progression. |
| Synonyms | CDCP1; CD318; SIMA135; TRASK; UNQ2486; PRO5773 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
