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CALCA/CGRP hFc Chimera, Human

Specific fragments of methylation changes in the target gene (Calca) were identified by IGV analysis. CGRP was applied to compare the effects on ASCs-T2DM morphology via phalloidin staining, proliferation through CCK-8 assay, and osteogenic differentiation with osteogenic staining, qPCR, and repair of calvarial defect. Furthermore, 5-azacytidine (5-az) was used to intervene ASCs-T2DM to verify the relationship between the methylation level of the target fragment and expression of Calca.
￥3000
Z05108-100

产品简介
Species Human
Protein Construction
CALCA/CGRP (Ala26-Asn141)
Accession # NP_001732.1		 hFc
N-term C-term
Purity > 95% as determined by Bis­Tris PAGE 
Endotoxin Level Less than 1EU per μg by the LAL method.
Expression System HEK293
Theoretical Molecular Weight 39.4 kDa
Apparent Molecular Weight Due to glycosylation, the protein migrates to 45-48 kDa based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles.
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图例
CALCA/CGRP HFc Chimera, Human

The purity of CALCA/CGRP hFc Chimera, Human is greater than 95% as determined by SEC-HPLC. »

CALCA/CGRP HFc Chimera, Human

CALCA/CGRP hFc Chimera, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

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技术背景
Target Background Specific fragments of methylation changes in the target gene (Calca) were identified by IGV analysis. CGRP was applied to compare the effects on ASCs-T2DM morphology via phalloidin staining, proliferation through CCK-8 assay, and osteogenic differentiation with osteogenic staining, qPCR, and repair of calvarial defect. Furthermore, 5-azacytidine (5-az) was used to intervene ASCs-T2DM to verify the relationship between the methylation level of the target fragment and expression of Calca.
Synonyms Calcitonin; CCP; PDN-21; CALCA; CALC1; CGRP; CGRP-I; CGRP1; CT; KC; PCT; Procalcitonin; Calcitonin 1; Katacalcin
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.