TSLP[Biotin], His & Avi, Human
¥5800 | |
Z04638-100 | |
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¥5800 | |
Z04638-100 | |
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Species | Human | ||||||
Protein Construction |
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Conjugate | Biotin | ||||||
Purity | > 95% as determined by BisTris PAGE | ||||||
Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
Biological Activity | Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch. | ||||||
Expression System | HEK293 | ||||||
Theoretical Molecular Weight | 17.8 kDa | ||||||
Apparent Molecular Weight | Due to furin cleavage site, the protein migrates to 12 kDa&25-30 kDa based on Bis-Tris PAGE result. | ||||||
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||||
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Immobilized TSLP[Biotin], His & Avi, Human, His Avi Tag at 1μg/ml(100μl/well) on the streptavidin precoated plate(5μg/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. »
Immobilized Anti-TSLP Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for TSLP[Biotin], His & Avi, Human, His Tag with the EC50 of 1.6ng/ml determined by ELISA. »
The purity of TSLP[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC. »
TSLP[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. »
Target Background | Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells. |
Synonyms | thymic stromal lymphopoietin; TSLP |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.