| ￥5800 | |
| Z04558-100 | |
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| Species | Human | ||||||
| Protein Construction |
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| Conjugate | Biotin | ||||||
| Purity | > 95% as determined by BisTris PAGE | ||||||
| Endotoxin Level | Less than 1EU per μg by the LAL method. | ||||||
| Biological Activity | Measured by its binding ability in a functional ELISA.Test result was comparable to standard batch. | ||||||
| Expression System | HEK293 | ||||||
| Theoretical Molecular Weight | 35.8 kDa | ||||||
| Apparent Molecular Weight | Due to glycosylation, the protein migrates to 60-68 kDa based on Bis-Tris PAGE result. | ||||||
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). | ||||||
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. | ||||||
| Storage & Stability | Upon receiving, the product remains stable for 6 months at -20℃ or below. Upon reconstitution, the product should be stable for 3 months at -80℃. Avoid repeated freeze-thaw cycles. |
Immobilized MICA[Biotin], His & Avi, Human, His Tag at 2 μg/ml (100 μl/well) on the streptavidin precoated plate(5 mg/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 59.7ng/ml determined by ELISA. »
Immobilized Anti-MICA Antibody, hFc Tag at 5 μg/ml (100 μl/Well) on the plate. Dose response curve for MICA[Biotin], His & Avi, Human, His Tag with the EC50 of 2.9 μg/ml determined by ELISA. »
The purity of MICA[Biotin], His & Avi, Human is greater than 95% as determined by SEC-HPLC. »
MICA[Biotin], His & Avi, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »
| Target Background | TMICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig‑like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin. |
| Synonyms | FLJ60820; MGC111087; MICA; PERB11.1; DAMA-345G11.2; FLJ36918; FLJ60820; MGC21250 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
