Enterokinase, His, Bovine

Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by a proline. rbEK with 6 × His-tag binds with Ni2+ affinity chromatography and was designed for removing from digestion system.

价格	￥400
产品编号	Z03004-100
规格	100 IU 500 IU 5000 IU 大单询价
数量

产品简介图例技术背景

产品简介

Source	P. pastoris
Species	Bovine
Purity	> 95% as analyzed by reducing SDS-PAGE.
Biological Activity	5 IU/μl.
Unit Definition	One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95% completion at 22°C in a buffer containing 25mM Tris-HCl, pH 8.0.
Endotoxin Level	< 1.0 EU/μg, determined by gel clot method.
Molecular Weight	~40 kDa, observed by reducing SDS-PAGE.
Formulation	Sterile liquid solution contians 20mM Tris, 200mM NaCl, 2mM CaCl₂, 50% glycerol, pH 7.4.
Components	100 IU(or 500IU or 5000IU) Recombinant Bovine Enterokinase (in 20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl₂, 50% glycerol) 100μg Cleavage Control Protein (Lyophilized after extensive dialysis against PBS, pH 7.0) 3.6 ml EK Dilution/Storage Buffer (20mM TrisHCl, pH 7.4, 200mM NaCl, 2mM CaCl₂, 50% glycerol) 1.8 ml 10X EK Cleavage/Capture Buffer (200mM Tris-HCl, pH 7.4, 500mM NaCl, 20mM CaCl₂)
Storage & Stability	Recombinant Bovine Enterokinase (rbEK) remains stable for 1 year at -20°C from date of receipt. It will remain stable at 37°C for one week without losing any activity. Please avoid freeze-thaw cycles.

图例

Lane 1: 2 μg of Enterokinase, His, Bovine, non-reducing (NR)
Lane 2: 2 μg of Enterokinase, His, Bovine, reducing (R)
> 95% as analyzed by SDS-PAGE »

技术背景

Synonyms

Enteropeptidase, ENTK, PRSS7

Target Background

Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by a proline. rbEK with 6 × His-tag binds with Ni²⁺ affinity chromatography and was designed for removing from digestion system.
Recombinant Bovine Enterokinase (rbEK) as the light chain is a single glycosylated polypeptide chain containing 200 amino acids. A fully biologically active molecule, rbEK has a molecular mass of 22.7 kDa and is obtained by proprietary chromatographic techniques at GenScript.

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.

澳门太阳游戏城app官网入口

Enterokinase, His, Bovine