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CFXpress™ E.coli Cell-free Protein Synthesis Kit

A ready-to-use, high-yield E. coli-based cell-free protein synthesis system with built-in T7 RNA polymerase for rapid expression from T7-driven DNA or mRNA templates

价格	￥675
产品编号	RP-D00005-1
规格	1 ml 5 ml 大单询价
数量

产品简介图例

Description

The GenScript CFXpress™ Cell-free E. coli Protein Synthesis Kit (CFPS) is a ready-to-use platform for rapid, high-yield in vitro protein expression from DNA or mRNA templates under the control of a T7 promoter.

The kit consists of three main components:
•Cell Extract
•Protein Synthesis Buffer
•Positive Control Plasmid

By following the provided protocol and combining Cell Extract and Protein Synthesis Buffer with your target templates, protein expression can be achieved within a few hours. The system supports both clonal and non-clonal DNA templates. Custom formulation guidance is included for expressing proteins with multiple disulfide bonds, promoting correct folding and improved functional yield. Synthesized proteins are compatible with downstream characterization, purification, and functional assays. The system is compatible with high-throughput automation workflows and dialysis method.

产品简介

Size	The kit is available in two sizes: 1 mL Kit: Suitable for 20 reactions (50 µL each) Includes: •Cell Extract x 2 Tubes •Protein Synthesis Buffer x 2 Tubes •Control Plasmid x 1 Tube 5 mL Kit: Includes: •Cell Extract x 5 Tubes •Protein Synthesis Buffer x 5 Tubes •Control Plasmid x 1 tube
Application	•Rapid production and screening of proteins such as antibody fragments, mini binders, mini proteins, and enzymes, supporting fast AI-driven design and testing cycles •Expression of membrane proteins •Express of cell-toxic proteins
Volume	Cell Extract: ~ 0.17 mL/ tube; Protein Synthesis buffer: ~ 0.26mL/ tube
Storage & Stability	- 80℃
Note	•Please find protocol and manual under "Document" section •For research use only

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CFXpress™ E.coli Cell-free Protein Synthesis Kit