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| RP-A00060-0.2 | |
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Cpf1 is a novel class of CRISPR-Cas DNA endonuclease. The LbCpf1/Cas12a allow efficient mutagenesis in zebrafish and Xenopus (Moreno-Mateos, et al. Nat Communications, 2017; 8: 2024). LbCpf1 increases homology-directed genome editing. |
| Form | Liquid |
| Concentration | 1mg/mL |
| Full mRNA length | 4063 nt |
| Full mRNA Molecular Weight | 1.32×10^6 Da |
| Storage buffer | 1mM Sodium citrate, pH 6.5 |
| Storage condition | Store at -20°C for short term (<3 months), store at -80°C for long term. |
| Appearance | Clear and free of foreign particles |
| RNA Length | Expected size band detected |
| RNA Content | Target ± 5% |
| Integrity | ≥ 75% |
| OD260/OD280 | 1.70 ~ 2.30 |
| Capping Efficiency | ≥ 90% |
| Endotoxin | < 10 EU/mg |
| pH | Target ± 0.5 |
| Transfect 2 μg of LbCas12a mRNA and 0.4 μg of TRAC crRNA into 5×104 HEK293T cells using Lipofectamine™ MessengerMAX™ (or equivalent), on day 3, extract the genomic DNA and analyze data by NGS sequencing. |
Editing efficiency of Cas12a on TRAC target in HEK293T cells. WT represents wildtype; X5 represents GenScript codon optimization algorithm. »
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
