| 询价 | |
| M00571 | |
|
|
|
|
|
|
|
|
|
| Product Description | Recombinant CHO-K1 cells stably overexpress Macaca fascicularis cytotoxic T-lymphocyte associated protein 4 (CTLA4) on the surface. The surface expression of cynomolgus CTLA4 is validated by FACS analysis. This cell line is recommended for cell-based binding assay to screen antibodies against CTLA4 or to measure binding affinity between CTLA4 and anti-CTLA4 antibodies. |
| Culture Properties | Adherent. |
| Stability | Stable through more than 16 passages without significant changes in assay performance or expression profile. |
| Size | Two vials of frozen cells (>1×106 per vial in 1 mL). |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Culture Medium | Ham’s F-12K (Kaighn’s), 10% FBS, 8 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies). |
| Complete Growth Medium | Ham’s F-12K (Kaighn’s) (Cat. No. 21127-022, Life Technologies), 10% FBS (Cat. No. 10099-141, Life Technologies) |
| Freeze Medium-DATA | 95% complete growth medium, 5% (V/V) DMSO (Cat. No. D2650, Sigma). |
Figure 1. FACS analysis of cell surface expression of cynomolgus CTLA4 on CHO-K1/cyno CTLA4 cells. The CHO-K1/cyno CTLA4 cells (blue) and the negative control CHO-K1 cells (red) were probed using PE-conjugated anti-human CD152 antibody (Cat. No. 557301, BD Biosciences).
»
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
