CHO-K1/Gα15/CCR5
Recombinant CHO-K1 cells stably overexpress human C-C motif chemokine receptor 5 (CCR5) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways.
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| M00523 | |
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| 询价 | |
| M00523 | |
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| Product Description | Recombinant CHO-K1 cells stably overexpress human C-C motif chemokine receptor 5 (CCR5) on the surface and contain high levels of G protein Gαi to couple with the receptor in downstream signaling pathways. |
| Culture Properties | Adherent. |
| Stability | Stable through more than 16 passages with no significant changes in assay performance or expression profile. |
| Size | Two vials of frozen cells (>1×106 per vial in 1 mL). |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Culture Medium | Ham’s F-12K (Kaighn’s), 10% FBS, 3 μg/ml Puromycin (Cat. No. A11138-03, Life Technologies), 100 μg/ml Hygromycin B (Cat. No. 10687010, Life Technologies) |
| Complete Growth Medium | Ham’s F-12K (Kaighn’s) (Cat. No. 21127, Life Technologies), 10% FBS. |
| Freeze Medium-DATA | 45% culture medium, 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma) |
Figure 1. CCL3-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/Gα15/CCR5 cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with CCR5 agonist, CCL3 (Cat. No. Z03137, Genscript). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and plotted against the log of the cumulative doses of CCL3 (mean ± SEM, n = 3). The EC50 of CCL3 on CHO-K1/Gα15/CCR5 cells was 0.28 μg/ml.
Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^ ((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is ∆RFU and starts at Bottom and goes to Top along a sigmoid curve.
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Figure 2. FACS analysis of cell surface expression of human CCR5 on CHO-K1/Gα15/CCR5 cells. The CHO-K1/Gα15/CCR5 cells (red) and the negative control CHO-K1 cells (blue) were probed using APC-conjugated anti-human CD195 antibody (Cat. No. 359122, Biolegend). »
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
