| 询价 | |
| M00319 | |
|
|
|
|
|
|
|
|
|
| Product Description | Recombinant HEK293 cells stably overexpress human 5-hydroxytryptamine receptor 2C (5-HT2C) on the surface and contain high level of G protein Gαi and Gαq to couple with the receptor in downstream signaling pathways. |
| Culture Properties | Adherent. |
| Stability | Stable through more than 16 passages with no significant changes in assay performance or expression profile. |
| Size | Two vials of frozen cells (>1×106 per vial in 1 mL). |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Culture Medium | DMEM, 10% FBS, 300 μg/ml Geneticin (Cat. No. 10131-035, Life Technologies) |
| Complete Growth Medium | DMEM (Cat. No. 10566, Life Technologies), 10% FBS |
| Freeze Medium-DATA | 45% culture medium, 45% FBS (Cat. No. 10099-141, Life Technologies), 10% DMSO (Cat. No. D2650, Sigma) |
Figure 1. 5-HT-induced concentration-dependent stimulation of intracellular calcium mobilization in HEK293/5-HT2C cells. The cells were loaded with Calcium-4 (Cat. No. R8142; Molecular Devices) prior to stimulation with 5-HT2C agonist, 5-HT (Cat. No. 14927, Sigma Aldrich). The intracellular calcium change was measured by FLIPRTETRA. The relative fluorescent units (RFU) were recorded and plotted against the log of the cumulative doses of 5-HT (mean ± SEM, n = 3). The EC50 of 5-HT on HEK293/5-HT2C cells was 0.29 nM.
Notes:
EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom) / (1+10^((LogEC50-X)*Hill Slope))
X is the logarithm of concentration. Y is the response.
Y is % stimulation of ∆RFU and starts at Bottom and goes to Top along a sigmoid curve. »
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
