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Enterokinase Animal-Free, His, Bovine

GenScript Enterokinase Animal-Free, His, Bovine is the light chain of recombinant bovine enterokinase that is a single glycosylated polypeptide chain containing 200 amino acids and is produced under animal free process, which is more friendly and safer for drug or vaccine development, manufacture and other applications. This product is designed with a 6 × His tag for further removing from the digestion system by being captured by Ni2+ affinity chromatography.
￥480
Z03747-100

产品简介
Source Recombinant Enterokinase expressed in P. pastoris
Species Bovine
Tag His Tag
Purity ≥ 95% as analyzed by SDS-PAGE
Biological Activity 5 U/μl
Unit Definition One unit is defined as the amount of enzyme sufficient to cleave 50 μg of control fusion protein (~24 kDa) in 16 hours to 95% completion at 22 °C in a buffer containing 20 mM Tris-HCl, 50 mM NaCl, 2 mM CaCl2 pH 7.4.
Endotoxin Level < 1.0 EU/μg of protein by gel clotting method
Molecular Weight Predicted MW: 27 kDa
Apparent MW: ~40 kDa, on SDS-PAGE under reducing conditions
Formulation Supplied as solution in 20 mM Tris-HCl, 200 mM NaCl, 2 mM CaCl2, 50% glycerol, pH 7.4.
Storage Buffer 20 mM Tris-HCl, 200 mM NaCl, 2 mM CaCl2, 50% glycerol, pH 7.4.
Storage & Stability Upon receiving, the product remains stable for 6 months at -20 °C. This product is stable for 1 week at 37 °C. Avoid repeated freeze-thaw cycles by making single-use aliquots before the solution is stored at -20 °C.
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应用指导
His Tag
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Enterokinase Animal-Free, His, Bovine

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技术背景
Target Background Enterokinase (EK) is a serine protease produced by cells of the duodenum, and is involved in mammalian digestion. It plays the role of turning trypsinogen to the active form of trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine at its cleavage site: Asp-Asp-Asp-Asp-Lys. Enterokinase does not function if the cleavage site is followed by a proline.
GenScript’s Enterokinase Animal-Free, His, Bovine is the light chain catalytic subunit of bovine enterokinase consisting of a single glycosylated polypeptide chain containing Ile801-His1035 (Accession # P98072). This recombinant protein is expressed in P. pastoris and purified to obtain high yields of the active enzyme. It is produced under an animal free process, and is suitable for drug and vaccine development, manufacture and other applications. This product is designed with a C-terminal 6 × His tag and can be removed after cleavage by Ni2+ affinity chromatography in resin (Cat. No. L00223) or magnetic beads (Cat. No. L00295).
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.