Incubate with 100 ng plasmid DNA at 37 °C for 30 min, tiny amount of GenScript DNase I can completely digest the DNA." onclick="clickMainImage(this)" src="/product/documents/down?doc_name=E00053_activity.jpg&file=scm_files/productFile_notes/2021/11/26/76845e4e-94e5-4f18-b600-f800fa791fba.jpg">
Incubate with 100 ng plasmid DNA at 37 °C for 30 min, tiny amount of GenScript DNase I can completely digest the DNA." data-src="/product/documents/down?doc_name=E00053_activity.jpg&file=scm_files/productFile_notes/2021/11/26/76845e4e-94e5-4f18-b600-f800fa791fba.jpg">

DNase I
Deoxyribonuclease I (DNase I) is DNA-specific endonuclease that cleaves both single-stranded DNA,double-stranded DNA and DNA-RNA hybrids, yielding 5'-phosphate-terminated polynucleotides with a free hydroxyl group on position 3' producing tetranucleotides. The activity of DNase I is strictly dependent on Ca2+ and can be activated by divalent metal ions such as Mg2+ or Mn2+. In the presence of Mg2+, DNase I nonspecifically recognizes and cleaves a double-stranded DNA at anysite on either strand, and in the presence of Mn2+, it recognizes and cleaves almost the same sites on both strands of the DNA to produce DNA fragments with blunt ends or sticky ends with 1~2 nucleotide overhangs. GenScript is offering DNase I produced by expression in a P. pastoris strain carrying a plasmid encoding the bovine DNase I.
¥180 | |
E00053-100 | |
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